Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation.
نویسندگان
چکیده
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 97 شماره
صفحات -
تاریخ انتشار 2015